Fractionation and analysis of neutral oligosaccharides by HPLC.
نویسندگان
چکیده
This unit describes the fractionation and analysis of neutral oligosaccharides by high-performance liquid chromatography (HPLC) on bonded amine columns. Separation is based upon hydrogen bonding between the NH2 groups of the column and the hydroxyl groups of the oligosaccharides. A support protocol describes the reduction and desalting of neutral oligosaccharides with sodium borohydride.
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ورودعنوان ژورنال:
- Current protocols in molecular biology
دوره Chapter 17 شماره
صفحات -
تاریخ انتشار 2001